Optical bioimaging

Visualising and quantifying biological systems

We use a range of optical microscopy techniques, many are fluorescence-based, to visualise and quantify the properties of biological systems.  We can examine:

• peptides and proteins
• mammalian and bacterial cells (2D and 3D cultures)
• multicellular systems (organoids and spheroids)
• model organisms and tissues across scales of length, time and complexity.

Typical applications include:

• Visualisation and analysis of the structural (dimensional) properties of biological systems
• Timelapse imaging of dynamic systems and processes (e.g. live mammalian and bacterial cells)
• Spatiotemporal imaging of systems under optogenetic control
• Intracellular uptake studies
• Protein and peptide dynamics and structure
• Functional imaging using fluorescent reporters (e.g. cell viability, calcium ion imaging)
• Co-localisation studies
• Measurement of the efficacy and mode of action of therapeutics (e.g. antimicrobials, chemotherapeutics)

These services are delivered using the following equipment:

• Confocal Laser Scanning Microscope (Leica Stellaris and Olympus FV-1000)
• Structured Illumination Fluorescence Microscope (custom-built)
• Airy beam light sheet microscope (Aurora, M Squared)
• Upright two photon microscope (custom-built)
• High performance computing systems for image analysis

We also have supporting facilities for cell culture and microbiology (Cat 2 laboratoires), sample preparation (mounting and fluorescent labelling) and optogenetics.

Don’t see what you are looking for? Our diverse skill set enables us to provide bespoke solutions. Please contact us to discuss your requirements.

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